Assessment of milk and blood enzymes in Holstein cows with subclinical mastitis

The objective of this study was to determine the activities lactate dehydrogenase (LDH), alkaline phosphatase (ALP), gamma-glutamyl transferase (GGT), aspartate aminotransferase (AST) and alanine (ALT) in milk blood lactating Holstein cows investigate their association with subclinical mastitis (SCM). This also aims evaluate impact SCM its severity based on California test (CMT) scores above mentioned enzymes, as well efficiency use an early predictive diagnostic tool. Thirty multiparous, dairy were examined using CMT according obtained results classified into four groups; control group 1 (SCM-free = 10, score 0), 2 (weak positive 8, +1), 3 (positive 7, +2) 4 (strong 5, +3). LDH, ALP, GGT significantly higher groups compared group. LDH activity mean ALP than 2. other groups. There a significant between enzymes (ALP GGT) cows. showed that causes considerable changes infected